Heparin-binding Protein (HBP)

HBP Antibody

Name	Anti-Human Heparin-binding protein (HBP) antibody
Catalog #	R439n4	R440e8	R441c3	R442e3	R152c7	R153a2	R236e3
Platforms Pairs	Chemiluminescence immunoassay (CLIA)				Immunochromatography assay (ICA)
	R439n4 (Capture) -R440e8 (Detection) R440e8 (Capture) -R153a2 (Detection) R152c7 (Capture) -R153a2 (Detection)				R442e3 (Capture) -R441c3 (Detection) R152c7 (Capture) -R236e3 (Detection)
Description	Rabbit monoclonal antibody, cultured in vitro				monoclonal antibody, cultured in vitro
Buffer	1xPBS，pH 7.4
Purity	Purity>95%, purified by Protein A/G chromatography
Storage	Aliquot and store at -20°C or lower. Avoid freeze / thaw cycles.

HBP Antigen

Name	Heparin-binding protein (HBP) antigen
Description	Recombinant, C-terminal His-tagged, in vitro expressed from mammalian cells
Applications	Calibrator and quality control product
Catalog #	K1523
Uniprot ID	P20160
Purity	>>90%, analyzed by R250-stained SDS-PAGE
Buffer	1xPBS，pH 7.4
Storage	Aliquot and store at -80°C. Avoid freeze / thaw cycles.
SDS-PAGE	Predicted MW around 25kDa (tagged)

Product Information

Heparin-bindingprotein (HBP) Antibody affinity

HBP Antigen K1523 was used to detect the rabbit HBP monoclonal antibody R439n4, R440e8, R441c3, R442e3 on ELISA platform, whose EC50 was 2.47ng/mL, 1.41ng/mL, 3.28ng/mL, 2.69ng/mL, respectively.

Fig.1 HBP antibody (R439n4) ELISA result

Fig.2 HBP antibody (R440e8) ELISA result

Fig.3 HBP antibody (R441c3) ELISA result

Fig.4 HBP antibody (R442e3) ELISA result

Acridinium Ester-labeled Chemiluminescence (AE-CLIA) platform

Correlation in clinical samples

R440e8-R153a2 matched antibody pair was verified in JOINSTAR^TM serum assigned samples (51 cases, concentration: 3.05-298.55ng/mL, clinical coefficient of determination R² > 0.98).

Fig.5 Correlation of HBP on CLIA platform in clinical samples

Sample No.	Concentration （ng/mL）	RLU
1	3.05	118238
2	4.16	131421
3	5.32	145199
...	...	...
50	287.91	2856594
51	298.55	3055494

Table.1 Correlation data in clinical samples

R439n4-R440e8 matched antibody pair was verified in JOINSTAR^TM serum assigned samples (51 cases, concentration: 3.05-298.55ng/mL, clinical coefficient of determination R² > 0.98).

Fig.6 Correlation of HBP on CLIA platform in clinical samples

Sample No.	Concentration （ng/mL）	RLU
1	3.05	20110
2	4.16	34839
3	5.32	50231
...	...	...
50	287.91	3565694
51	298.55	4039815

Table.2 Correlation data in clinical samples

Time-resolved immunofluorescence (TRFIA) platform

Correlation in clinical samples

R440e8-R153a2 matched antibody pair was verified in JOINSTAR^TM serum assigned samples (21 cases, concentration: 0-298.55ng/mL, clinical coefficient of determination R² > 0.95).

Fig.7 Correlation of HBP on CLIA platform in clinical samples

Sample No.	Concentration （ng/mL）	T/C
1	0	0.0128
2	7.22	0.1453
3	9.28	0.1489
...	...	...
50	287.91	1.1946
51	298.55	1.4773

Table.3 Correlation data in clinical samples

Diagnostic Significance of Heparin-binding Protein (HBP) Antigen

Heparin-binding protein (HBP), also known as azurocidin or cationic antibacterial protein (CAP37), is a multifunctional protein with bactericidal function and chemotactic characteristics, which mainly exists in the secretory granules of neutrophils and azurophilic granules.

Diagnostic indicators for early-stage bacterial infections

Heparin-binding protein (HBP) is the first marker increasing in the process of bacterial infection. When infection occurs, bacteria invade blood vessels, stimulating neutrophils to release HBP, thus increases the content of HBP in plasma.

HBP alters the permeability of vascular endothelial cells through its strong positive charge, inducing plasma leakage and tissue edema (a characteristic manifestation of inflammatory response). In addition, HBP activates monocytes and macrophages to release inflammatory mediators such as tumor necrosis factor-α (TNF-α) and γ-interferon (INF-γ) and thus enhancing the body's immune response to infection or injury.
Compared with other conventional infection indicators such as PCT, CRP, IL-6 and leukocyte count, the variation of HBP appears earlier in infection with higher sensitivity, specificity, positive/negative detection rates and other comprehensive advantages. Dynamic monitoring of HBP concentration in plasma can be used to evaluate the level of sepsis and predict the risk of shock or cardiac arrest in severely infected patients. Long in short, HBP has great indicative significance to early diagnosis and treatment of clinical bacterial infections.

Reference Value

Normal: < 10 ng/mL
Infected: 20 - 30 ng/mL
Severely infected: 100 - 1000 ng/mL
Critical:> 1000 ng/mL

References

[1] Linder A, Akesson p, Inghammar M, et al. Elevated plasma levels of heparin-binding protein in intensive care unit patients with severe sepsis and septic shock [J]. Crit Care, 2012, 16(3): R90.
[2] Dankiewicz J , Linder A, Annborn M, et al. Heparin-binding protein: an early indicator of critical illness and predictor of outcome in cardiac arrest [J]. Resuscitation, 2013, 84(7): 935-939.

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